serum metabolomic profiles  (Metabolon Inc)

Journal: bioRxiv

Article Title: Gut bacterial lactate stimulates lung epithelial mitochondria and exacerbates acute lung injury

doi: 10.1101/2025.03.24.645052

Figure Lengend Snippet: (a) The complete pathway for L– Lactate production from the B. adoelscentis BD1 genome. B. adolescentis BD1 encodes genes required for all chemical transformations. Gene symbols shown in green italics. Select intermediate and end products are displayed in black. (b) Liquid chromatography-mass spectrometry-based metabolomic profiling of B. adolescentis conditioned media plotting -log 10 ( p -value) against log 2 (fold change) (log 2 FC). Predicted annotations were conducted by MSDIAL, and the negative ionization feature MZ 89.02, Retention time 2.371 minute predicted to be Lactate is annotated and colored in green. n=3 per group. (c) The lactate feature (MZ 89.02, Retention time 2.371 minutes) peak area is plotted for BHI or B. adolescentis conditioned media. (d) L -lactate production by enzymatic assay for B. adolescentis grown in vitro for 24 hours (n=3 biological replicates per group). (e) L -lactate levels in cecal contents from GF, B. adolescentis colonized, or E. lenta colonized mice (n=4 mice per group). (c-e) The red dashed line indicates the detection limit. The p -values indicate t- tests between the indicated groups. (f-j) Bioenergetic profiling of BEAS-2B cells pretreated with L -lactate at two concentrations. (e) Extracellular acidification rate (ECAR) normalized to 1000 cells or (f) Oxygen consumption rate (OCR) normalized to 1000 cells is plotted against time. Asterisks in (f-g) indicate Wilcox p <0.05 for the concentration of the indicated compound versus the 0 μM control group for a given timepoint. (h) Basal respiration, (i) Maximal Respiration, (j) Spare Respiratory Capacity for L -lactate treated BEAS-2B cells. (f-j) Each experiment had 4-8 replicates per group in an experimental plate, with 2-4 replicates done for each compound.

Article Snippet: We re-analyzed published serum metabolomic profiles of 198 patients enrolled in the E arly A ssessment of R enal and L ung Injury (EARLI) intensive care unit patient cohort .

Techniques: Liquid Chromatography, Mass Spectrometry, Production Enzymatic Assay, In Vitro, Concentration Assay, Control

Journal: bioRxiv

Article Title: Gut bacterial lactate stimulates lung epithelial mitochondria and exacerbates acute lung injury

doi: 10.1101/2025.03.24.645052

Figure Lengend Snippet: (a) The full-length B. adolescentis genome was input to the Distilled and Refined Annotation of Metabolism (DRAM v0.1.2) tool. The outputted metabolism summary indicates whether and which specific metabolic conversions are predicted to be present within the genome of B. adolescentis . (b) Untargeted metabolomic profiling of B. adolescentis conditioned media compared to BHI. Compound category is indicated by point color. Compounds are separated based on whether compounds could be annotated by MSDIAL (Known) or could not be annotated (Unknown). n=3 independent conditioned media specimens per group.

Article Snippet: We re-analyzed published serum metabolomic profiles of 198 patients enrolled in the E arly A ssessment of R enal and L ung Injury (EARLI) intensive care unit patient cohort .

Techniques: